Tijjani RG
Universiti Kebangsaan Malaysia, Malaysia
Title: Biochemical investigation of the fracture healing properties of Marantodes Pumilum Var. Alata in postmenopausal osteoporotic rats
Biography
Biography: Tijjani RG
Abstract
This study aims to investigate, in ovariectomized rats, the changes in bone biochemical markers induced by MPva leaf extract in an attempt to further evaluate its fracture-healing property. Thirty (30) healthy female Sprague-Dawley rats were sorted into five groups (n=6) namely: Sham-operated (SO); Ovariectomized control (OC); Estrogen treatment (ET); 20 mg leaf treatment (MP20); and 100 mg leaf treatment groups (MP100). All rats, except the SO group, were ovariectomized to induce a state of menopause. Eight weeks post-ovariectomy, the right tibiae of rats were fractured and fixed with titanium plates. For another 8 weeks, after osteotomy, the ET group was treated with 64.5μg/kg/d estrogen (Premarin®) while the MP20 and MP100 groups were treated with 20mg and 100 mg/kg/d oral doses of MPva leaf extract, respectively. Before and after treatment, blood sample was collected from rats for assay of inflammatory markers while, after treatment, fractured tibiae were harvested from euthanized rats for assay of bone turnover and anti-oxidant markers using ELISA. At p < 0.05, results were considered significant. Serum level of osteocalcin was significantly elevated (p < 0.05) in MPv100 group while pyridinoline level was significantly elevated in all treatment groups as well as the OC. Post-treatment serum levels of IL-6 were significantly higher in MPv20 and MPv100 while TNF-alpha was significantly higher in MPv100 group compared to pre-treatment levels (p < 0.05). Levels of GPx and SOD in tibia were significantly elevated (p < 0.05) in both MPv20 and MPv100. Results obtained revealed that the bone-repair effects of MPva leaves could be exerted by its ability to enhance bone remodelling activities due to its anti-oxidant and inflammation modulatory properties.